Cphycocyanin cpc is a phycobiliprotein found in blue green algae, such as spirulina platensis, is often used as a dietary nutritional. After ammonium sulphate precipitation the purity of phycocyanin was 1. In spirulina species, phycocyanin pigments increase from 12% to 22% on increasing nitrogen levels. The extraction using ultrasonic bath in the presence of glass pearls in the biomass proved to be the most efficient method, 56% higher than using freezing and thawing the method most frequently used, and presented a extraction yield of 43. Comparison of different extraction methods for phycocyanin extraction and yield from spirulina platensis s.
Phycocyanin not stable against ph, temperature, light and humidity 3,4. Phyocyanin extraction from microalgae spirulina platensis. Recent research suggests that phycocyanin is light sensitive, so phycocyanin must be preserved in the dark 43. However, the current bioprocessing method that involves the extraction of large amounts of phycocyanin from cells is cumbersome. To increase the extraction yield, intensification techniques may be used as pretreatment or simultaneously with the extraction. Stable isolation of phycocyanin from spirulina platensis. In this study we demonstrate the production of thermostable phycocyanin from the unicellular red alga galdieria sulphuraria. By considering various significant biological applications of cpc, the aim of this study was extraction and purification of cphycocyanin from the dry spirulina powder using various. Isolation and characterization of thermostable phycocyanin.
Extraction using sonication by water proved to be an efficient method. The pellet was suspended in 100 ml of 20 mm acetate buffer containing 50 mm sodium chloride and 0. Quantitative analysis of cphycocyanin from spirulina pacifica low temperature method background. Choose to extract a set of specific pages as one pdf or as separate pdfs. The yield of the extraction was defined as silveira et al. Cphycocyanin extraction from spirulina platensis wet biomass. Abs 620 absorbance of the sample at 620 nm and abs 652 absorbance at 652 nm. The invention relates to a method for extracting and stabilising phycocyanin from at least one material containing same. A simple method for extracting cphycocyanin from spirulina. Comparison of different extraction methods for phycocyanin. The use of this low cost substrate increases the cell concentration by 60 % during 8 days cultivation to reach 0. Cphycocyanin cpc, a phycobiliprotein, is one of the key pigments of spirulina, a microalgae used in many countries as a dietary supplement. Extraction and estimation of c phycocyanin a 500 ml of homogenized log phase 15 days old culture was centrifuged at 4,000 rpm to obtain pellet. A method for stably purifying a functional dye, phycocyanin from.
Cpcv yield db 2 where yield is the extraction yield of phycocyanin in mg of cphycocyanindry biomass g, v is the solvent volume ml and db is the dry biomass g. Said method is characterised in that same includes a step of macerating the material or materials in glycerol or a waterglycerol mixture. Extraction and purification of cphycocyanin from dry. Under the optimum conditions, phycocyanin with a purity of 3. Pdf phycocyanin extraction in spirulina produced using. Results showed that %ee was significantly affected p spirulina. A faster rate was obtained with higher temperatures.
In the present study an attempt has been made to develop a simple and efficient method for the extraction employing liquid nitrogen for cell disruption followed by enzymatic extraction of the phycocyanin. Phycocyanin is a highly valuable pigmented protein synthesized by several species of cyanobacteria and red alga. The enzymolysis method using lysozyme was carried out under various enzyme concentrations, extraction time and temperature. All product pictures are for display purposes only, it may not reflect the exact product color or mesh size, please refer to spec sheet for details. Extraction and purification of cphycocyanin from dry spirulina. From product literature, assuming phycocyanin sensor. Extraction and purification of cphycocyanin from spirulina.
Sivasankari, naganandhini and david ravindran department of biology, gandhigram rural institutedeemed university, dindigul, tamilnadu, india corresponding author abstract introduction spirulina is a bluegreen algae owing to the. C for a complete 100% phycocyanin extraction in triscl buffer ph 8. Instead, phycobiliproteins aggregate to form clusters that. Factors affecting phycocyanin extraction from spirulina. The extraction using ultrasonic bath in the presence of glass pearls in the biomass proved to be the most efficient method, 56% higher than using freezing and. Although a number of reports are available for the extraction and puri. It was found that the best condition for ultrasonic frequency was 45 khz, with an extraction time of 15 minutes and a volume sample 50 g. The htl biocrude oil obtained from the original spirulina feedstock and the residual after the cpc extraction were compared. Click the select a file button open a pdf you want to extract pages from in the open dialog box, select the bodea. The extraction was clean and efficient, and the purity and. Development of a novel method for the purification of cphycocyanin. All phycobiliproteins are watersoluble, so they cannot exist within the membrane like carotenoids can. Cpcv yield db 2 where yield is the extraction yield of phycocyanin in mg of c phycocyanin dry biomass g, v is the solvent volume ml and db is the dry biomass g. How to extract pages from a pdf adobe acrobat dc tutorials.
Alternative methods for analysis of cyanobacterial. Phycocyanin is a pigment protein complex from the lightharvesting phycobiliprotein family, along with allophycocyanin and phycoerythrin. Click split pdf, wait for the process to finish and download. The algal slurry was neither washed nor centrifuged. Quantitative analysis of cphycocyanin from spirulina. Algal pigments have massive commercial value as natural colorants in nutraceutical, cosmetics, and pharmaceutical industries, besides their health benefits. In addition to these health benefits, this pigment has been used in dietary nutritional supplements and natural colorant applications in the food, nutraceutical, cosmetic, and. Select the pages you want to extract from the pdf by clicking on them individually, or by typing the page numbers into the page selection box. Extraction and estimation of cphycocyanin a 500 ml of homogenized log phase 15 days old culture was centrifuged at 4,000 rpm to obtain pellet.
The purified cyanobacterial isolate was subcultured in bg11 broth. A simple method for efficient extraction and purification of. Avila jerley, prabu dm 2015 purification, characterization and antioxidant properties of cphycocyanin from spirulina platensis scrutiny journal 2. Crude pc obtained was purified using activated charcoal and chitosan, to go along with ammonium sulphate precipitation.
We are currently experiencing intermittent display of pdf links on some article pages. Extraction methods for phycocyanin determination in. Phycocyanin extraction is carried out from wet or dry biomass. Optimization of phycocyanin extraction from spirulina. The extraction, isolation and purification of cphycocyanin have been studied for many years, resulting in diverse methodologies with a range of yields and grades of purity. Therefore fresh wet biomass was suitable for phycocyanin extraction. Phycocyanin was extracted by repeated freezethaw cycles and purified in a twostep process using am.
These statements have not been evaluated by the food and drug administration. Study of phycocyanin production from spirulina platensis. This product is not intended to diagnose, treat, cure or prevent any disease. Evaluation of different extraction methods for phycocyanin.
Jul 16, 2014 extraction and purification of phycocyanin, was completed in four major steps. The evaluation of the extraction, based on the concentration of phycocyanin, was set by equation 3, deduced by bennett and bogorad 1973. The obtained data indicated that phycocyanin was preferentially partitioned into the ilsrich phase and the atps composed of 23% ww bmimcland 29% ww kh 2 po 4 at 30c and ph 7. Factors affecting phycocyanin extraction from spirulina platensis. Algae phycocyanin,production, uses and market oilgae oil.
Cphycocyanin cpc was extracted from fresh spirulina platensis by deploying a species of nonpathogenic nitrogenfixing bacteria, namely, klebsiella pneumoniae. For the latter, select the pages you wish to extract. C phycocyanin cpc, a phycobiliprotein, is one of the key pigments of spirulina, a microalgae used in many countries as a dietary supplement. Wo2014045177a1 method for extracting and stabilising. Spirulina platensis was developed by a hexane extraction process. Choose to extract every page into a pdf or select pages to extract. The effects of different biomass forms and extraction solvents on the phycocyanin content ww during the extraction of phycocyanin from s. An extensive range of pigments including phycobiliproteins are present in algae.
C phycocyanin cpc was extracted from fresh spirulina platensis by deploying a species of nonpathogenic nitrogenfixing bacteria, namely, klebsiella pneumoniae. Microencapsulation is one of an alternative to process phycocyanin into powder, which has a particle size between 1 m 5. Using realtime monitoring and phycocyanin probes to track. Phycocyanin extraction, spirulina platensis, bluegreen algae a wide range of phycocyanin extraction methods were studied for the processing of spirulina biomass. Methods for extraction, isolation and purification of c. Extraction and separation of phycocyanin from spirulina using. Phycocyanin is a nontoxic, watersoluble pigment protein from microalgae that exhibits antioxidant, antiinflammatory, hepatoprotective, and neuroprotective effects. Cell debris was removed by centrifugation at 5,000 rpm for 10 min and the extract thus obtained was termed as crude extract. Free service for documents up to 200 pages or 50 mb and 3 tasks per hour. Extraction and purification of phycocyanin, was completed in four major steps. Paper open access related content physical characteristics of. Spirulina and other bluegreen algae contain cphycocyanin, which acts as an accessory pigment when light energy is captured and transferred to chlorophyll a. Separate one page or a whole set for easy conversion into independent pdf files.
Extraction of phycocyanin from spirulina plantesis using. Aug 08, 2019 extraction using sonication by water proved to be an efficient method. Shinde2 1center for biotechnology, pravara institute of medical sciences du, loni4736, maharashtra, india. Further extraction of phycocyanin pc showed that temperature greatly contributed to extraction efficiency ee. This work first showed that very high amounts of phycocyanins, such as 11. If you are unable to see the pdf link please follow the. The evolution of the phycocyanin concentration for various temperatures is shown in fig. Previous studies on the extraction of pc from wet biomass of spirulina platensis 2526and anabaena oryzae 27have demonstrated the efficacy of the sft method, where optimal temperatures for freezing. Organization instrument excitation nm emission nm massdep turner designs databank datalogger with cyclops 7 probe phycocyanin sensor 590 650 us epa ysi multisensor probesondes 565605. Extraction and purification of c phycocyanin from dry spirulina powder and evaluating its antioxidant, anticoagulation and prevention of dna damage activity suresh p. Pdf cphycocyanin extraction from spirulina platensis wet biomass. Drying methods were resulted in approximately 50% loss of phycocyanin.
The extraction temperature varied from 30 to 60 c to evaluate its effect on the extraction efficiency of phycocyanin at a ph of 7. Solidliquid extraction of phycocyanin from spirulina. Recent developments in production and biotechnological. Effect of ultrasonic extraction on production and structural. Compiled by a diverse team of experts, with experience in scientific and industrial fields, the comprehensive report for wastewater treatment using algae is the first report that provides indepth analysis and insights on this important field. By considering various significant biological applications of cpc, the aim of this study was extraction and purification of c phycocyanin from the dry spirulina powder using various. The extraction of phycobilliproteins involves cell rupture and release of these proteins from within the cell. Phycocyanin has potential as a natural dye, but the effort required to maintain phycocyanin during storage. The residual biomass after cpc extraction was converted into biocrude oil via hydrothermal liquefaction htl. Wait a few moments for our pdf splitter to split your pdf pages.